Journal: Nature biomedical engineering

Article Title: Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens

doi: 10.1038/s41551-017-0084

Figure Lengend Snippet: a, An illumination light sheet enters the bottom surface of a tissue sample at an oblique 45-deg angle (purple). The specimen(s) is placed on a modular glass-plate sample holder, which is inserted into a two-axis translation stage and scanned in a serpentine pattern of volumetric image strips to enable 3D imaging over a large lateral extent. Fluorescence emission (cyan), which is generated along the light sheet, is collected in the orthogonal direction by an objective lens. The fluorescence signal is then transmitted through an emission filter (green) and a dual-channel image splitter (for 2 color imaging) before being imaged onto a high-speed sCMOS camera. b , To provide aberration-free imaging, a solid immersion lens (SIL) and oil layer are used for refractive-index matching of both the illumination and collection beams into and out of the glass plate and tissue sample. c , As the sample is translated in the primary scanning direction, x , oblique 2D light-sheet images with a width, w , and adjustable height, h , are captured in succession to form a 3D imaging volume. d , In contrast to conventional microscopy methods that have a shallow fixed depth of focus and slow 3D imaging rates, the deep depth of focus and adjustable vertical field of view of the open-top light-sheet microscope makes it optimal for both rapid microscopy of irregular/tilted tissue surfaces, and deep volumetric microscopy of clinical specimens. The imaging speeds shown correspond to acquiring single-channel images with height, h .

Article Snippet: A dual-channel image-splitting module (W-VIEW GEMINI, Hamamatsu Photonics, Japan) is used to image two fluorescence imaging channels onto two halves of a high-speed sCMOS detector array with 2048 × 2048 pixels (ORCA Flash v2.0, Hamamatsu Photonics, Japan).

Techniques: Imaging, Fluorescence, Generated, Refractive Index, Microscopy

Journal: Biomedical Optics Express

Article Title: Selective plane illumination microscope dedicated to volumetric imaging in microfluidic chambers

doi: 10.1364/BOE.455377

Figure Lengend Snippet: Experimental set-up with L: laser, M: Mirror, DL: Diverging Lens, CL: Converging Lens, Cyl.L: Cylindrical Lens, MC: Microfluidic Chip, S: (manual & computed-controlled) micromertric stages, DO: Detection Objective, F: Filter, TL: Tube Lens, C: sCMOS Camera. A) Simplified sketch of the SPIM. B) Picture of the real set-up with in inserts: the yellow rectangle for the view of the laser source placed just behind the breadboard, and the blue rectangles for the view of the afocal system to enlarge the laser beam.

Article Snippet: The tube lens is used to form the image of the fluorescent structure onto a high-sensitive sCMOS camera (HPF ORCA FLASH 4.0V3-Pack, Hamamatsu).

Techniques: